Equinatoxins, pore-forming proteins from the sea anemone Actinia equina, belong to a multigene family.
نویسندگان
چکیده
The multigene family of equinatoxins, pore-forming proteins from sea anemone Actinia equina, has been studied at the protein and gene levels. We report the cDNA sequence of a new, sphingomyelin inhibited equinatoxin, EqtIV. The N-terminal sequences of natural Eqt I and III were also determined, confirming two isoforms of EqtI, differing at position 13. The number of Eqt genes determined by Southern blot hybridization was found to be more than five, indicating that Eqts belong to a multigene family.
منابع مشابه
Isolation and characterization of three lethal and hemolytic toxins from the sea anemone Actinia equina L.
Lethal and hemolytic toxins were purified by acetone precipitation, Sephadex G-50, CM-cellulose and CM-Sephadex column chromatography from the tentacles and bodies of the sea anemone Actinia equina. The isolated toxins, with a mol. wt of 19,000 determined by SDS-polyacrylamide gel electrophoresis, differed only slightly in amino acid composition and had a high tryptophan content. The isoelectri...
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ورودعنوان ژورنال:
- Toxicon : official journal of the International Society on Toxinology
دوره 37 10 شماره
صفحات -
تاریخ انتشار 1999